Amplicon coverage report metrics

The amplicon coverage report includes the following metrics:

Metric

Description

Region

The amplicon name as defined in the BED file for the amplicon region.

Chr

Chromosome

Start

The start coordinate of the amplicon.

End

the end coordinate of the amplicon.

Attributes

The attribute in the BED file for the amplicon.

TotalE2E

The number of assigned reads from end to end for the amplicon region.

FwdE2E

The number of assigned forward strand reads from end to end for the amplicon region.

RevE2E

The number of assigned reverse strand reads from end to end for the amplicon region.

Total

The total number of reads assigned to this amplicon. This value is equal to Fwd + Rev.

Fwd

The number of forward strand reads assigned to this amplicon. This value may differ from the reads for end to end.

Rev

The number of reverse strand reads assigned to this amplicon. This value may differ from the reads for end to end.