Download an amplicon coverage report

You can download an amplicon coverage report in Ion Reporter™ Software. Amplicon coverage reports contain details such as amplicon coverage, chromosome location, and start and end positions.

In the Analysis tab, click Overview.
Select the row of the analysis that you want to report on.
Click (Actions)View Coverage Report.
Click Export to generate a tab-separated CSV file that contains details such as chromosome location, and start and end positions on forward and reverse strands.

The downloaded file is named ampliconmetrics.csv.

Amplicon coverage report metrics

The amplicon coverage report includes the following metrics:


Metric	Description
Region	The amplicon name as defined in the BED file for the amplicon region.
Chr	Chromosome
Start	The start coordinate of the amplicon.
End	the end coordinate of the amplicon.
Attributes	The attribute in the BED file for the amplicon.
TotalE2E	The number of assigned reads from end to end for the amplicon region.
FwdE2E	The number of assigned forward strand reads from end to end for the amplicon region.
RevE2E	The number of assigned reverse strand reads from end to end for the amplicon region.
Total	The total number of reads assigned to this amplicon. This value is equal to Fwd + Rev.
Fwd	The number of forward strand reads assigned to this amplicon. This value may differ from the reads for end to end.
Rev	The number of reverse strand reads assigned to this amplicon. This value may differ from the reads for end to end.