Read number impact on calculation sensitivity

  • At above 150,000 mapped reads, the analysis workflow is very sensitive.

  • Between 20,000 and 150,000 mapped reads, the more reads, the more sensitive the analysis workflow is.

  • With 20,000 or few mapped reads, we recommend that you rerun the sequencing experiment, if possible. At around 20,000 or fewer reads, the potential for false negatives (missing a real fusion) increases.