Example RNA Exon Variant graph
You can view bar graphs that
illustrate intragenic exon rearrangements in Ion Reporter™ Software.
RNA Exon Variant
graphs show exon variant read counts normalized to the wild-type. Specific exons and exon
variants are shown along the X-axis. In wild-type assays shown along the x-axis, entries are
labeled with a gene ID followed by a sequence of adjacent exons. For example, wild-type exons
six and seven of the epidermal growth factor receptor gene is represented as EFGR.E6E7. The
Y‑axis represents the read counts for each variant, normalized to the wild type.
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Example analysis where an RNA exon 2–7 deletion occurred in the EFGR gene. Normalized read counts for the EFGR variant that contains the intragenic fusion (EGFR-EGFR.E1E8.DelPositive.2) are higher relative to the normalized read counts for a wild type EFGR assay (EFGR.E6E7).
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Example analysis where exon-skipping (i.e., exon deletion) of exon 14 in the MET gene was detected. Normalized read counts for the variant representative of the exon-skip event (MET‑MET.M13M15.1) are higher relative to the MET wild-type assays (for example, MET.M13M14M15.ENST00000397752.WT and MET.M17M18M19M20.ENST00000397752.WT).
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