Example RNA Exon Tile Fusion Imbalance plots for a single analysis

RNA Exon Tile Fusion Imbalance plots provide a visual representation of the RNA fusion imbalance results for the detection of novel fusions in a partner agnostic manner. This plot shows the imbalance scores and p‑values for genes in the selected analysis result, including the control gene, genes that are imbalance-positive, and genes that are imbalance-negative.

In the plot, dashed gray lines mark the threshold for an imbalance call. The blue quadrant is at a fixed position, with the imbalance score set at 2 and the –log(p-value) set at 1.3. The plot provides a visual estimate for positive imbalance calls. This image shows that NTRK1 is an exon tile fusion imbalance positive gene in the selected analysis. The plotted position of some fusion positive genes do not appear within the blue (positive) quadrant. Genes denoted with are exon tile fusion imbalance positive, such as RET, although RET is not plotted within the blue quadrant. This is because the fusion imbalance threshold for RET is lower than the fixed quadrant that is displayed. The blue quadrant is a guideline. Refer to the symbol displayed in the plot for the fusion imbalance call.
Table 1. Graph symbols

Symbol

Description

Fusion‑positive genes

Fusion‑negative genes

Control genes