V-gene usage and number of clones plot example
IGH V-gene usage and number of clones
Select Number of Clones for a view of a spectratyping plot that highlights the number of clones that are identified for each variable gene-CDR3 nucleotide length combination.
This is an example of an IGH V-gene usage and number of clones spectratyping plot from an Oncomine™ BCR IGH‑LR Assay analysis. Circle color indicates the number of clones having a particular variable gene-CDR3 nucleotide length combination. Specific variable genes may more frequently participate in VDJ recombination, leading to an enrichment in distinct clones for those variable genes. You can further partition the clones by isotype in Ion Reporter™ Software, with the buttons that are above the interactive spectratyping plot.