Immune repertoire analysis results report isotype representation within a sample, which is
calculated through either the number of reads per isotype, the number of clones per
isotype, or the number of lineages per isotype. This view is available only for the
Oncomine™ BCR IGH‑LR Assay. Select
Isotype usage
from the Views
list to see the graphs that represent the data.
Ordering of the isotypes reflects their position within the IGH locus. B cells of plasmablast or plasma cell type express the BCR at a higher level than memory or naïve B cells. Therefore these cell types have an outsized contribution to the reads per isotype plot, but less so for calculations made at the clone or lineage level.
