Upload a target regions BED file workflow preset

You can upload a target regions BED file from a copied analysis workflow, then create a target regions BED file workflow preset for use in other analysis workflows.

IMPORTANT! Use only BED file names that do not contain spaces. If a file name includes spaces, the analysis fails.

  1. In the Workflows tab, click Create.
  2. In the Research Application column, click DNA. Click any type of sample group.
  3. For Reference, select GRCh38 or hg19.
  4. In the Target Regions section, click Upload.
  5. In the Upload Target Regions File dialog box:
    1. Select your library type or technology.
    2. Click Select File , then browse to your BED file.
    3. Ensure that the correct BED file name appears in the display field, then click Upload.

      Ion Reporter™ Software uploads and verifies your BED file. When verification is complete, the progress bar changes to green, and a message confirms that the files are ready for use.

    4. Click Close.

    The new BED file preset appears in the Target Regions section of the Reference step.

  6. You can now upload additional BED files, continue to create an analysis workflow, or cancel the analysis workflow creation.

    In the Workflows tab, click Presets, then select Annotation Sets > Target Regions Files. The new BED file is listed in the Workflow Presets table.

  7. (Optional) When you have run a set of samples to validate that your preset works as you intended, select the preset in the Workflow Presets table, then click Actions > Lock to lock the analysis workflow.

    You cannot undo a lock action.