Reference output files for Metagenomics

The metagenomics reference FASTA output files may have 2 formats:

• The FASTA line starts with the text ">MG and is followed by primerset , primerName, direction , count , percentage ,Phylum , Class , Order , Family , Genus , Species separated by "|" .

  The following line is an example:

  
  >MG|1|V2|R|27|97.72|Proteobacteria|Epsilonproteobacteria|Campylobacterales|Helicobacteraceae|Helicobacter|pylori|

• The FASTA line starts with the text ">MG and is followed by count, percentage , Phylum , Class , Order , Family , Genus , Species separated by "|".

  The following line is an example:

>MG|187|99.1|Bacteroidetes|Bacteroidia|Bacteroidales|Bacteroidaceae|Bacteroides|vulgatus|

For details about the FASTA file, see the Metagenomics 16S algorithms overview white paper at https://tools.thermofisher.com/content/sfs/brochures/ion-reporter-16s-metagenomics-algorithms-whitepaper.pdf.

For more information about metagenomics, visit the Ion 16S™ Metagenomics product page on Connect at https://www.thermofisher.com/us/en/home/life-science/sequencing/dna-sequencing/microbial-sequencing/microbial-identification-ion-torrent-next-generation-sequencing/ion-16s-metagenomics-solution.html.