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Sample Name
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The name of the sample, as it was entered into the Planned Run for Torrent Suite™ Software.
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Barcode
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The name of the specific barcode in the barcode kit that was used for the sequencing run.
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Mapped Reads
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The total number of reads that were mapped to the reference in Torrent Suite™ Software.
Reads that are not uniquely mapped can have equally well-aligned reads that are mapped to
multiple locations. Such reads are typically mapped randomly to
one location.
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On Target
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The total number of reads that were mapped in the target regions file to any targeted region of the reference in Torrent Suite™ Software.
A read is on target only if at least
one aligned base overlaps with a target region. For example, a
read is not counted if the read overlaps a targeted region on a
region that includes a flanking sequence that is aligned. In
this case the read is not counted due to poor matching of 5'
bases.
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Mean Depth
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The summary statistics for reads that are assigned to specific amplicons. Each sequence
read is assigned to exactly one of the amplicons specified by
the targets file. Reads are assigned to particular amplicon
targets based on whether the (5') mapping location is
sufficiently near the end of the amplicon region, taking into
account the read direction (mapping strand).
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Uniformity
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The percentage of bases in all targeted regions (or whole‑genome) that is covered by at least 20% of the average base coverage depth reads.
Cumulative coverage is linearly interpolated between nearest integer base read depths.
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