Transcripts in REFSEQ and ENSEMBL canonical transcripts file

In UCSC curated canonical transcripts set, there are some discrepancies when multiple transcripts are used for a given gene. The shorter transcripts have been removed and the transcript with the appris_principal_1 tag is retained in the Ion Reporter™ Software REFSEQ canonical transcript set. This tag is used by gencode to mark the primary transcript of a specific gene. If the appris_principal_1 tag is missing, the longest transcript is used so that there is just one canonical transcript for a given gene. If two transcripts are the same length, Ion Reporter™ Software includes both genes and lists them in alphabetical order.

The UCSC transcripts for the following genes are replaced with the transcripts in the New transcript column in Ion Reporter™ Software:

Gene

Current transcript

New transcript

FGFR2

NM_022970.3

NM_000141.5

FGFR3

NM_001163213.1

NM_000142.5

ABL1

NM_007313.2

NM_005157.6

ARAF

NM_001256196.1

NM_001654.5

CHEK2

NM_001005735.1

NM_007194.4

GNAS

NM_080425.2

NM_000516.7

TP53

NM_001276760.1

NM_000546.6