Version—PolyPhen-2 v2.1.0r367, using the stand-alone default installation instructions and usage
BLAST version—2.2.25
Protein database—Uniref100, downloaded on August 12, 2011 from ftp://ftp.uniprot.org/pub/databases/uniprot/current_release/uniref/uniref100/uniref100.fasta.gz