Exon tile fusion baselines
When an exon tile fusion baseline is applied to an analysis workflow, Ion Reporter™ Software can model the amplicon expression variation within a gene. The exon tile fusion baseline is used to normalize a test sample, correct the coverage in exon-tile amplicons, and identify the presence or absence of expression imbalance in a gene.
Ion Reporter™ Software includes predefined exon tile fusion baselines. You can also create an exon tile fusion baseline workflow preset in the software. You can later add the exon tile fusion baseline to an analysis workflow and use it as a baseline control for analyses.
Note: A sample can qualify for inclusion in a baseline of one gene but can be excluded from the baseline of another gene. For example, if a gene is not expressed in the tissue of the sample, the sample is excluded for that gene.
Genes that are included in exon-tiling imbalance analysis workflows. Each row summarizes the imbalance baseline composition status of a gene.
Each sample is represented by a column. In this example, sample 24 is included in the baseline for ALK, FGFR2, and HMBS, but excluded for NTRK1, NTRK2, NTRK3, and RET.
The last column, with the header total, lists the total number of valid baseline samples per gene.
When a baseline results in fewer than 5 valid samples for one or more genes, a warning message is shown. In this example, the warning message applies to ALK.