Example V-gene Mutation plots
The following examples are V-gene Mutation plots for analyses from three different immune repertoire analysis workflows:
Select V-gene Mutation for a view of a spectratyping plot that highlights the frequency of mutated bases over the variable gene of identified clones.
IGH V-gene usage and mutation rate
This is an example of an immunoglobulin heavy chain (IGH) spectratyping plot from an Oncomine™ BCR IGH‑LR Assay analysis. Circle color indicates the average frequency of mutated bases for clones having a particular variable gene-CDR3 nucleotide length combination. B cells that have undergone isotype switching tend to have a higher frequency of somatic hypermutation than B cells expressing IgM or IgD isotypes, which tend to represent naive B cells. Systematic differences with respect to reference may indicate the presence of polymorphism within the variable gene that is not captured by the IMGT database. You can further partition the clones by isotype in Ion Reporter™ Software, with the buttons that are above the interactive spectratyping plot.
IGK V-gene usage and mutation rate
This is an example of an immunoglobulin light chain kappa (IGK) spectratyping plot from an Oncomine™ BCR IGKL‑SR Assay analysis. The plot highlights the frequency of mutated bases over the variable gene of identified clones. Flanking region mutations that occur in the intron between the joining genes and the constant gene are shown in the immunoglobulin kappa constant intron (IGKCint) column. The circle color indicates the average frequency of mutated bases for clones that have a particular variable gene-CDR3 nucleotide length combination.
IGL V-gene usage and mutation rate
This is an example of an immunoglobulin light chain lambda (IGL) spectratyping plot from an Oncomine™ BCR IGKL‑SR Assay analysis. The plot highlights the frequency of mutated bases over the variable gene of identified clones. The circle color indicates the average frequency of mutated bases for clones that have a particular variable gene-CDR3 nucleotide length combination.
TRG V-gene usage and mutation rate
This is an example of a T-cell receptor gamma (TRG) spectratyping plot from an Oncomine™ TCR Gamma‑SR Assay analysis. The plot highlights the frequency of mutated bases over the variable gene of identified clones. The circle color indicates the average frequency of mutated bases for clones that have a particular variable gene-CDR3 nucleotide length combination.