hg19 and GRCh38 human genome references
In Ion Reporter™ Software you can use human genome references hg19 or GRch38 for either predefined or user-defined analysis workflows. You can use the Ion GRch38 human reference when you create analysis workflows for panels that are designed based on this reference. The GRch38 reference genome in Ion Reporter™ Software is based on the latest GRC human reference assembly and is the first major update since 2009. Highlights include changes to chromosome coordinates, fixed errors in the former sequence, addition of mitochondria, and multiple loci for some highly variable genes.
Ion AmpliSeq™ Designer currently offers the GRch38 human reference and related target reference (BED) file for designs based on the GRch38 reference.
Note: If you edit earlier versions of analysis workflows that use the hg19 reference genome, you cannot change the reference genome to Ion GRch38.