Analysis workflow templates for Ion AmpliSeq™ HD
Ion Reporter™ Software supports predefined analysis workflow templates for use with Ion AmpliSeq™ HD panels. The Ion AmpliSeq™ HD predefined analysis workflow templates must be activated by an administrator before use. To activate the templates, contact Technical Support or the support representative. After activation, select the template appropriate for the application and use it to create a user-defined analysis workflow. For more information, see Copy and edit an analysis workflow template.
IMPORTANT! Ensure that the analysis workflow template name that you use matches the type of Ion AmpliSeq™ HD panel files that you include in the analysis. For example, if you use panel files for a liquid biopsy fusions single sample, copy the Ion AmpliSeq™ HD for Liquid Biopsy - w2.6 - Fusions - Single Sample analysis workflow template to create the user-defined analysis workflow.
Use the following guidelines to find Ion AmpliSeq™ HD analysis workflow templates or analysis workflows that have been copied in the Workflows tab.
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To filter for Ion AmpliSeq™ HD analysis workflow templates, or copy and edited Ion AmpliSeq™ HD analysis workflows that include saved panel files, search for Target: AmpliSeq HD.
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To filter for Tumor Ion AmpliSeq™ HD analysis workflow templates, use a combination of the Workflow filters Research Category: Oncology – Solid Tumor, and Target: AmpliSeq HD.
Note: Workflow versions 2.7 are released with Ion Reporter™ Software 5.22 for use with the Ion 560™ Chip.
|
Research Application |
Analysis workflow template |
Description |
|---|---|---|
|
HD for Tumor (DNA) |
Ion AmpliSeq™ HD for Tumor - w2.7 - DNA - Single Sample |
Detects and annotates low frequency (to 0.5% limit of detection) variants (SNPs, INDELs and CNVs) from targeted DNA libraries using Ion AmpliSeq™ HD technology. Compatible with DNA that is purified from tumor samples. |
|
HD for Tumor (Fusions) |
Ion AmpliSeq™ HD for Tumor - w2.7 - Fusions - Single Sample |
Detects and annotates gene fusions from targeted RNA libraries using Ion AmpliSeq™ HD technology. Compatible with RNA that is purified from tumor samples. |
|
HD for Tumor (DNA+Fusions) |
Ion AmpliSeq™ HD for Tumor - w2.7 - DNA and Fusions (Single Library) - Single Sample |
Detects and annotates low frequency (to 0.5% limit of detection) variants (SNPs, INDELs, CNVs and fusions) from targeted nucleic acid libraries using Ion AmpliSeq™ HD technology. Compatible with DNA and RNA that is purified together from tumor samples. |
|
HD for Tumor (DNA+Fusions) |
Ion AmpliSeq™ HD for Tumor - w2.7 - DNA and Fusions (Separate Libraries) - Single Sample |
Detects and annotates low frequency (to 0.5% limit of detection) somatic variants (SNPs, INDELs and CNVs) from targeted DNA libraries, and gene fusions from matching targeted RNA libraries using Ion AmpliSeq™ HD technology. Compatible with DNA and RNA that is purified separately from tumor samples. |
|
HD for Liquid Biopsy (DNA) |
Ion AmpliSeq™ HD for Liquid Biopsy - w2.7 - DNA - Single Sample |
Detects and annotates low frequency (to 0.5% limit of detection) variants (SNPs, INDELs and CNVs) from targeted DNA libraries using Ion AmpliSeq™ HD technology. Compatible with DNA that is purified from cell-free liquid biopsy samples. |
|
HD for Liquid Biopsy (Fusions) |
Ion AmpliSeq™ HD for Liquid Biopsy - w2.7 - Fusions - Single Sample |
Detects and annotates gene fusions from targeted RNA libraries using Ion AmpliSeq™ HD technology. Compatible with RNA that is purified from cell-free liquid biopsy samples. |
|
HD for Liquid Biopsy (DNA+Fusions) |
Ion AmpliSeq™ HD for Liquid Biopsy - w2.7 - DNA and Fusions (Single Library) - Single Sample |
Detects and annotates low frequency (to 0.5% limit of detection) variants (SNPs, INDELs, CNVs and fusions) from targeted nucleic acid libraries using Ion AmpliSeq™ HD technology. Compatible with DNA and RNA that is purified together from cell-free liquid biopsy samples. |
|
HD for Liquid Biopsy (DNA+Fusions) |
Ion AmpliSeq™ HD for Liquid Biopsy - w2.7 - DNA and Fusions (Separate Libraries) - Single Sample |
Detects and annotates low frequency (to 0.5% limit of detection) somatic variants (SNPs, INDELs and CNVs) from targeted DNA libraries, and gene fusions from matching targeted RNA libraries using Ion AmpliSeq™ HD technology. Compatible with DNA and RNA that is purified separately from cell-free liquid biopsy samples. |