Transcripts in REFSEQ and ENSEMBL canonical transcripts file

In UCSC curated canonical transcripts set, there are some discrepancies when multiple transcripts are used for a given gene. The shorter transcripts have been removed and the transcript with the appris_principal_1 tag is retained in the Ion Reporter Software REFSEQ canonical transcript set. This tag is used by gencode to mark the primary transcript of a specific gene. If the appris_principal_1 tag is missing, the longest transcript is used so that there is just one canonical transcript for a given gene. If two transcripts are the same length, Ion Reporter Software includes both genes and lists them in alphabetical order.

The UCSC transcripts for the following genes are replaced with the transcripts in the New transcript column in Ion Reporter Software:

Gene

Current transcript

New transcript

FGFR2

NM_022970.3

NM_000141.5

FGFR3

NM_001163213.1

NM_000142.5

ABL1

NM_007313.2

NM_005157.6

ARAF

NM_001256196.1

NM_001654.5

CHEK2

NM_001005735.1

NM_007194.4

GNAS

NM_080425.2

NM_000516.7

TP53

NM_001276760.1

NM_000546.6