MSI analysis results

You can use the analysis workflows for Oncomine™ Comprehensive Assay Plus to determine whether microsatellite instability (MSI) is present. You can apply parameters that detect MSI markers in these analysis workflows, to identify a form of genomic instability in the replication of repetitive DNA.

The Oncomine Comprehensive Plus - 550 - w3.2 – DNA (and DNA and Fusions) analysis workflow uses the RMC control and a system-installed baseline for analyses; the Oncomine Comprehensive Plus - 560 - w3.2 - DNA (and DNA and Fusions) workflow uses the MSI On-Chip control sample with RMC control.

The RMC control captures sample and library preparation variations. The on-Chip Control, which is generally a designated normal sample on the chip, is used to help determine sequencing based variations. The MSI signal generated by the on-chip control is used to evaluate the MSI signal generated for all DNA samples that are sequenced on the same chip to generate an MSI score. With use of the MSI on-chip control samples, the addition of RMC controls is still required during the library preparation.

To view MSI analysis results, see Visualize MSI analysis results.