Import panel files from AmpliSeq.com

You can import files for Ion AmpliSeq and Ion AmpliSeq HD Made-to-Order, On-Demand, or some Ready-to-Use, and Oncomine tumor specific research panels from AmpliSeq.com into Ion Reporter Software during analysis workflow creation. For more information, see Ion AmpliSeq and Ion AmpliSeq HD panel types.

Only ordered and quoted panel files are available for import into Ion Reporter Software.

You must have an access code to sign in to AmpliSeq.com when you import custom panel files. For more information, see Generate an access code for AmpliSeq.com.
  1. In the Research Application step, select an appropriate research application and sample group for the panel, then click Next.
  2. In the Reference step, import a target regions file.

    Option

    Description

    Custom Panel

    1. Under Target Regions, click AmpliSeq Import.

    2. In the Import for AmpliSeq dialog box, select the Custom Panel tab for Ion AmpliSeq or Ion AmpliSeq HD Made-to-Order panel files, or Ion AmpliSeq On Demand panel files.

    3. Enter an account user name.
    4. In the Password field, enter the access code. For more information, see Generate an access code for AmpliSeq.com.

    5. Click List My AmpliSeq Panels.
    6. Select the panel files that you want to import from the dropdown list, then click Import.

    Fixed Panel

    1. Under Target Regions, click AmpliSeq Import.

    2. In the Import for AmpliSeq dialog box, select the Fixed Panel tab for Community or Ready-to-Use panel files.

    3. From the list, select the panel file that you want to import.

    4. Click Import.

  3. If the analysis includes fusions, you can import fusion panel files.

    Option

    Description

    Custom Panel

    1. Under Fusion Panel, click AmpliSeq Import.

    2. In the Import for AmpliSeq dialog box, select the Custom Panel tab for Ion AmpliSeq or Ion AmpliSeq HD Made-to-Order, or Ion AmpliSeq On Demand fusion panel files.

    3. Enter an account user name.

    4. In the Password field, enter the access code. For more information, see Generate an access code for AmpliSeq.com.

    5. Click List My AmpliSeq Panels.

    6. From the list, select the fusion panel file that you want to import, then click Import.

    Fixed Panel

    1. Click AmpliSeq Import under the Fusion Panel field.

    2. In the Import for AmpliSeq dialog box, select the Fixed Panel tab for Community or Ready-to-Use panel files.

    3. Enter an account user name.

    4. In the Password field, enter the access code. For more information, see Generate an access code for AmpliSeq.com.

    5. Click List My AmpliSeq Panels.

    6. From the list, select the fusion panel file that you want to import.

    7. Click Import.

  4. Complete the remaining steps for analysis workflow creation.

    For more information, see Create a new analysis workflow.

The target regions files and fusion panel files are added to the list of files that can be selected in the Reference workflow step.

Generate an access code for AmpliSeq.com

Before you start this procedure, you need to have a valid AmpliSeq.com account.

Access codes have been added to AmpliSeq.com to improve security and compatibility with single sign-on features. The access codes increase security because they are unique to each AmpliSeq.com account.

You must generate an access code and sign in with the access code each time that you import files for Ion AmpliSeq and Ion AmpliSeq HD Made-to-Order, On-Demand, or some Ready-to-Use research panels from AmpliSeq.com into Ion Reporter Software. The imported files are used when you create an analysis workflow for use with the AmpliSeq.com panels.

You need to generate an access code for the AmpliSeq.com account just once, if you save the access code when it is generated. If you do not save the access code, you can regenerate a new one when you sign in again on AmpliSeq.com.

You need to set a new access code when the code expires.

  1. Go to AmpliSeq.com, and sign in to the account.
  2. Click My AccountManage Access Code.
  3. Generate an access code.
    1. In the Manage Access Code dialog box, select an Access Code Expiration in the dropdown list.
    2. Click Generate.
    3. Click Copy to Clipboard.
      The access code is available for use on the clipboard.
    4. (Optional) Click Save to save the access code for use when you return to AmpliSeq.com.
You can now use the access code to import panel files from AmpliSeq.com. For more information, see Import panel files from AmpliSeq.com.